Sci. Adv. Today 1 (2015) 25226  
  Research Article  
   
   
         
Rolling circle transcription by T7 RNA polymerase on a DNA catenane template without promoter sequence  
  Qi Li, Xing-Yu Wang, Sheng Liu, Guang-Qi Wu, Jing Wang, Xing-Guo Liang and Wei Wu  
     
College of Food Science and Engineering, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, China
   
  Abstract  
  It has been reported that T7 RNA polymerase can initiate and carry out rolling circle transcription (RCT) on a circular DNA template even if no promoter sequence was present. Although this unexpected phenomenon was believed to be related to the “bubble-like” structure during transcription, no reasonable explanation was proposed. Here we synthesized several single-stranded DNA catenanes with controlled linking number (Lk) to mimic the transcription bubble in vivo. The RCT results showed that transcription efficiency on the catenanes consisting of two circles was much better than that on a single DNA circle. Obviously, it was proved that “bubble-like” structure helped the transcription. The effect of the topology (Lk) of DNA catenanes on the transcription was also discussed.  
     
   
  Cite this article as:  
  Qi Li, Xing-Yu Wang, Sheng Liu, Guang-Qi Wu, Jing Wang, Xing-Guo Liang and Wei Wu , Rolling circle transcription by T7 RNA polymerase on a DNA catenane template without promoter sequence, Sci. Adv. Today 1 (2015) 25226.